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Description: Our IFN-γ ELISA kit is designed for precise and reliable quantification of interferon gamma (IFN-γ) in human samples. This high-sensitivity kit ensures accurate cytokine detection in serum, plasma, and cell culture supernatants, making it ideal for immune response and inflammation research.
Product NameHuman IFN-γ(Interferon gamma) ELISA KitAliasInterferon gamma ELISA Kit, IFN-gamma ELISA Kit, Immune interferon ELISA Kit, IFNG ELISA KitCatalogue No.EH0164Size48T/96TSpeciesHumanUniProt IDP01579Sample Type血清，血浆，细胞培养上清液，细胞裂解液或组织裂解液，其它生物液体样品Detection MethodSandwich ELISA, Double AntibodyDetection WavelengthOD450Reaction Duration4小时Range15.
625-1000pg/ml (1pg=20mIU)Sensitivity9.375pg/mlStorage2-8°C(Sealed), Don't cryopreserve.
SpecificitySpecifically binds with IFN-γ , no obvious cross reaction with other analogues.ELISA Kit Components
Kit Components — Item — Size(48T) — Size(96T) — Storage Condition for Opened Kit
E001 — ELISA Microplate(Dismountable) — 8×6 — 8×12 — Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002 — Lyophilized Standard — 1vial — 2vial — Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E003 — Biotin-labeled Antibody(Concentrated, 100X) — 60ul — 120ul — 2-8°C (Avoid Direct Light)
E034 — HRP-Streptavidin Conjugate(SABC, 100X) — 60ul — 120ul
E024 — TMB Substrate — 5ml — 10ml
E039 — Sample Dilution Buffer — 10ml — 20ml — 2-8°C
E040 — Antibody Dilution Buffer — 5ml — 10ml
E049 — SABC Dilution Buffer — 5ml — 10ml
E026 — Stop Solution — 5ml — 10ml
E038 — Wash Buffer(Concentrated, 25X) — 15ml — 30ml
E006 — Plate Sealer — 3 pieces — 5 pieces
E007 — Product Description — 1 copy — 1 copy
Required Instruments and Reagents
Assay Procedure Summary
Standard Curve
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining.
After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) — OD-1 — OD-2 — Average
0 — 0.033 — 0.035 — 0.034
15.625 — 0.097 — 0.102 — 0.099
31.25 — 0.173 — 0.182 — 0.177
62.5 — 0.266 — 0.279 — 0.271
125 — 0.534 — 0.561 — 0.545
250 — 0.844 — 0.887 — 0.861
500 — 1.441 — 1.514 — 1.47
1000 — 2.18 — 2.291 — 2.224
Recovery
Add a certain amount of IFN-γ into the sample. Calculate the recovery by comparing the measured value with the expected amount of IFN-γ in the sample.
Sample Type — Recovery Range(%) — Average(%)
serum(n=10) — 90-99 — 97
EDTA plasma(n=10) — 87-100 — 95
Heparin plasma(n=10) — 85-105 — 97
Linearity
Dilute the sample with a certain amount of IFN-γ at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type — 1:2 — 1:4 — 1:8
serum(n=10) — 86-104% — 90-105% — 85-102%
EDTA plasma(n=10) — 84-99% — 84-98% — 83-98%
Heparin plasma(n=10) — 84-100% — 82-91% — 81-85%
Precision(%)
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item — Intra-assay Precision — Inter-assay Precision
Sample — 1 — 2 — 3 — 1 — 2 — 3
n — 20 — 20 — 20 — 20 — 20 — 20
Mean (pg/ml) — 31.47 — 130.3 — 510 — 32 — 123.1 — 501
Standard deviation — 1.68 — 7.53 — 29.12 — 1.7 — 6.94 — 26.45
CV(%) — 5.35 — 5.78 — 5.71 — 5.32 — 5.64 — 5.28
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) — 37°C for 1 month — 2-8°C for 6 months
Average(%) — 80 — 95-100
