Double-stranded RNA (dsRNA) ELISA Kit (J2 based)

The Croyez Double-stranded RNA (dsRNA) ELISA Kit is a highly sensitive and quantitative immunoassay developed based on the double-antibody sandwich ELISA platform. It enables accurate detection of dsRNA contaminants commonly present in in vitro transcribed (IVT) mRNA or mRNA vaccine formulations.

Residual dsRNA is an unwanted by-product during IVT synthesis and is known to trigger innate immune responses. Therefore, monitoring and minimizing dsRNA levels is essential for ensuring mRNA product quality, safety, and translational efficiency.

This kit is specifically optimized for use in mRNA drug development, QC release testing, and process optimization, providing a robust tool for detecting trace levels of dsRNA in a variety of sample types.

DsRNA in the sample binds to this solid phase, then reacts with HRP-conjugated mouse anti-dsRNA monoclonal antibody. After washing away unbound antibody, a substrate solution is added, and the reaction is measured by absorbance at 450 nm.

DsRNA levels are quantified by comparing sample absorbance to a standard curve.

Platform:
Pre-coated 96 Microplate (12 x 8 well strips)

Sensitivity:
Limit of detection (LoD): 0.

288 ng/mL
Limit of quantification (LoQ): 1.694 ng/mL.

Detection Range:
1.562-100 ng/mL

Reactivity:
Double-stranded RNA(dsRNA)

Components:
Double-stranded RNA (dsRNA) ELISA plate
Standard
Standard & Sample diluent buffer
HRP-antibody conjugate
HRP-antibody conjugated diluent buffer
20 X wash buffer
TMB
Stop solution
Microplate sealing film

Storage:
Store at 2-8°C.

Usage:
For Research Use Only

Shipping Conditions:
Blue ice