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ELK Biotechnology CO.,Ltd. 鄂Cor(Cortisol) ELISA Kit from ELK Biotechnology CO.,Ltd. 鄂. High-quality elisa product for research applications.
Product name: — Cor(Cortisol) ELISA Kit
Reactivity: — General
Alternative Names: — Hydrocortisone
Assay Type: — Competitive Inhibition
Sensitivity: — 0.93 ng/mL
Standard: — 200 ng/mL
Detection Range: — 3.13-200 ng/mL
Sample type: — serum, plasma, urine and other biological fluids
Assay length: — 2h
Research Area: — Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: — This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cor.
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cor.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added.
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm.
The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve.
