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BPS BioscienceSortase A Assay Kit from BPS Bioscience. The Sortase A Assay Kit comes in a convenient 96-well format, with enough purified recombinant Sortase A, Triglycine, Abz/DNP substrate, and assay buffer...
Assay Kit Format
Fluorogenic
Species
Human
Supplied As
The Sortase A Assay Kit comes in a convenient 96-well format, with enough purified recombinant Sortase A, Triglycine, Abz/DNP substrate, and assay buffer for 96 enzyme reactions.
Format
Catalog # — Name
71086 — Sortase A, His-Tag*
79938 — 2x Sortase assay buffer
79939 — Triglycine
79940 — Abz/Dnp substrate
79685 — Low binding, black NUNC 96-well plate
*The concentration of Sortase A is lot-specific and will be indicated on the tube containing the enzyme.
Materials Required But Not Supplied
UniProt #
Q9S446
Background
Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall, maintaining bacterial virulence and infectivity. Sortase A cleaves a specific peptide sequence (LPXTG recognition motif) within a target protein between threonine and glycine.
The cysteine residue of the active site forms a transient thioacyl intermediate complex with the substrate protein. This intermediate complex and substrate is then immediately attacked by oligo-glycine nucleophiles present on peptide-glycans of the bacterial wall to form an amide bond.
Since Sortase A is a critical enzyme needed to maintain the infectivity of the bacterium, it represents a promising therapeutic target. Especially for the treatment of infections caused by antibiotic-resistant bacteria.
References
1. Mazmanian, S.
K., et al.
1999. "Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall.
" Science 285(5428): 760-763. 2.
Spirig, T., et al.
. 2011.
"Sortase enzymes in Gram‐positive bacteria." Molecular microbiology 82(5): 1044-1059.
