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ELK Biotechnology CO.,Ltd. 鄂Human CDK1(Cyclin Dependent Kinase 1) ELISA Kit from ELK Biotechnology CO.,Ltd. 鄂. High-quality cell signaling pathway product for research applications.
Product name: — Human CDK1(Cyclin Dependent Kinase 1) ELISA Kit
Reactivity: — Human
Alternative Names: — CDC2; CDC28A; CDK1; CDC28-A; CDKN1; P34CDC2; p34 protein kinase; Cell Division Control Protein 2 Homolog; Cell Division Cycle 2,G1 To S And G2 To M
Assay Type: — Sandwich
Sensitivity: — 0.066 ng/mL
Standard: — 10 ng/mL
Detection Range: — 0.16-10 ng/mL
Sample type: — Tissue homogenates, cell lysates and other biological fluids.
Assay length: — 3.5h
Research Area: — Signal transduction;Enzyme & Kinase;Tumor immunity;Neuro science;
Uniprot ID: — P06493
Test principle: — The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDK1.
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain Human CDK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm.
The concentration of Human CDK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
