RNase Inhibitor - recombinant, L pack

RNase inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNase A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity (4 x 10-14M).

It does not inhibit the RNases I, T1, T2, H, U1, U2 and Cl3. In addition, RNase inhibitor shows no inhibition of polymerase or reverse transcriptase activity and so can be used for cDNA synthesis and in one-step RT-PCR reactions.

The murine version of RNase inhibitor lacks the pair of cysteines identified in human version,therefore it has significantly improved resistance to oxidation.

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PRODUCT DETAILS


Source:

Mus musculus, recombinant


Concentration:

40 units/µl


Formulation:

liquid (Supplied in 20 mM HEPES-KOH pH 7.5, 50 mM KCl, 8 mM DTT and 50 % [v/v] glycerol)


Application Notes:

RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume.

The application of RNase inhibitor is recommended for:

cDNA synthesis / RT-PCR
In vitro transcription/translation
RNA purification
RNA protection assays
Separation and identification of specific ribonuclease activities
Other applications where the integrity of RNA is essential




Long Term Storage:

-20°C


Background / Technical Information:

For the Original Manufacturer's data sheet please click here.


Regulatory Status:

RUO - Research Use Only — PRODUCT DETAILS — Source: — Mus musculus, recombinant — Concentration: — 40 units/µl — Formulation: — liquid (Supplied in 20 mM HEPES-KOH pH 7.

5, 50 mM KCl, 8 mM DTT and 50 % [v/v] glycerol) — Application Notes: — RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume.

The application of RNase inhibitor is recommended for:

cDNA synthesis / RT-PCR
In vitro transcription/translation
RNA purification
RNA protection assays
Separation and identification of specific ribonuclease activities
Other applications where the integrity of RNA is essential — Long Term Storage: — -20°C — Background / Technical Information: — For the Original Manufacturer's data sheet please click here. — Regulatory Status: — RUO - Research Use Only — TECHNICAL DOCUMENTS
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PRODUCT DETAILS

Source: — Mus musculus, recombinant

Concentration: — 40 units/µl

Formulation: — liquid (Supplied in 20 mM HEPES-KOH pH 7.5, 50 mM KCl, 8 mM DTT and 50 % [v/v] glycerol)

Application Notes: — RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume. The application of RNase inhibitor is recommended for:

cDNA synthesis / RT-PCR
In vitro transcription/translation
RNA purification
RNA protection assays
Separation and identification of specific ribonuclease activities
Other applications where the integrity of RNA is essential

Long Term Storage: — -20°C

Background / Technical Information: — For the Original Manufacturer's data sheet please click here.

Regulatory Status: — RUO - Research Use Only

TECHNICAL DOCUMENTS
Print as PDF — RELATED PRODUCTS
No related products available

PRODUCT SUPPORT
Technical Service
Bulk Quantity Quote
Customer Service — CATALOG SECTION

Size :

5x2000 U