Mybiosource, USA

Human IL-1alpha ELISA Kit

Name: Human IL-1alpha ELISA Kit
Brand: Mybiosource, USA
SKU: MBS355312-96

Human IL-1alpha ELISA Kit from Mybiosource, USA. 96-well plates.

สอบถามราคา

SKUMBS355312-96

Catalog #MBS355312-96

หมวดหมู่

Cytokine

รายละเอียดสินค้า

Introduction

Interleukin-1 alpha (IL-1α) is a protein of the interleukin-1 family that in humans is encoded by the IL1A gene which spans 10.2 kb and has 7 exons.

It is 1 of 2 structurally distinct forms of IL1, the other being IL1B, this two proteins are synthesized by a variety of cell types, including activated macrophages, keratinocytes, stimulated B lymphocytes, and fibroblasts, and are potent mediators of inflammation and immunity.

IL1A may play a role in the genesis of inflammation by augmenting the transcription of proinflammatory genes, a mechanism not affected by extracellular inhibitors. IL1A has been administered to patients during receiving autologous bone marrow transplantation.

Principle of the Assay

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1α polyclonal antibody was pre-coated onto 96-well plates.

And the biotin conjugated anti-IL-1α polyclonal antibody was used as detection antibodies.

The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed