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Croyez Bioscience Co., Ltd.BspQI Restriction Enzyme is a Type IIS restriction endonuclease that recognizes a specific DNA sequence and cleaves outside its recognition site. It is widely used in DNA mapping, genomic analysis, molecular biology workflows, and genome characterization studies.
BspQI can recognize non-palindromic sequences and cleave outside the recognition site. The recognition sequence of BspQI is 5'-GCTCTTCN1/N4-3', and it is utilized for plasmid digestion to produce poly(A/T/G/C)-terminated linearized DNA fragments with specific cohesive ends.
The product is provided in liquid form with optimized reaction buffer containing albumin to enhance enzyme stability, ensuring optimal enzyme performance.
BspQI has become particularly important in large-scale genome characterization and optical genome mapping workflows, where unique sequence motifs are used to generate genomic fingerprints. The enzyme enables accurate structural variation analysis, genome assembly validation, and characterization of complex genomic regions.
