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EXBIO Praha A.S., Czech RepublikAnti-Ki-67 Alexa Fluor 488. Analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
Mouse IgG1
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.
4 ml) is sufficient for 100 tests. Intracellular staining.
Cow, Human
Nuclei of the Hodgkin lymphoma cell line L428
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells.
It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Fluorochromes (additional)
1. A4-155-T100 Anti-Ki-67 Alexa Fluor® 488
100 tests
2. A6-155-T100 Anti-Ki-67 Alexa Fluor® 647
100 tests
3. 1P-155-T100 Anti-Ki-67 PE
100 tests
4. T7-155-T100 Anti-Ki-67 PE-Cy™7
100 tests
5.11-155-C100 Anti-Ki-67 Purified
0.1 mg
