WPMY-1 Cells

General information
Description The depositor reports that the RWPE-1 cell line, derived from the same prostate, was screened for Hepatitis B
and C, and human immunodeficiency viruses, and was found to be negative.
Organism Human
Tissue Prostate, stroma
Synonyms WPMY1
Characteristics
Age 54 years
Gender Male
Morphology Myofibroblast
Growth
properties
Adherent
Identifiers / Biosafety / Citation
Citation WPMY-1 (Cytion catalog number 305083)
Biosafety level 1
Expression / Mutation
Receptors
expressed
Androgen receptor, expressed
Protein
expression
Fibronectin, Smooth Muscle Alpha-Actin, Vimentin
Antigen
expression
kallikrein 3, KLK3(prostate specific antigen, PSA), Homo sapiens
Tumorigenic No
CLS Cell Lines Service GmbH | Dr.-Eckener-Str. 8 | 69214 Eppelheim | Germany
Tel.: +49(0)6221 405780 | www.cytion.com | info@cytion.com
1
Product sheet
WPMY-1 | 305083
Handling
Culture
Medium
DMEM
Medium
supplements
10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
Passaging
solution
Accutase
Subculturing Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-
10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet
must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells
with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks
which contain fresh medium.
Split ratio 1:2 to 1:4
Fluid renewal 2 to 3 times per week
Freeze
medium
CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of
cryopreserved
cultures
The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate
culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate
culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius
water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As
soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain
and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions.
Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the
cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend
the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be
omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the
cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further
steps are described in the subculture sectio