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CLS - Cell Lines Service, GermanyWPMY-1 Cells from CLS - Cell Lines Service, Germany. 5 ml.
General information Description The depositor reports that the RWPE-1 cell line, derived from the same prostate, was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.
Organism Human Tissue Prostate, stroma Synonyms WPMY1 Characteristics Age 54 years Gender Male Morphology Myofibroblast Growth properties Adherent Identifiers / Biosafety / Citation Citation WPMY-1 (Cytion catalog number 305083) Biosafety level 1 Expression / Mutation Receptors expressed Androgen receptor, expressed Protein expression Fibronectin, Smooth Muscle Alpha-Actin, Vimentin Antigen expression kallikrein 3, KLK3(prostate specific antigen, PSA), Homo sapiens Tumorigenic No CLS Cell Lines Service GmbH | Dr.-Eckener-Str.
8 | 69214 Eppelheim | Germany Tel.: +49(0)6221 405780 | www.
cytion.com | info@cytion.
com 1 Product sheet WPMY-1 | 305083 Handling Culture Medium DMEM Medium supplements 10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.
5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate Passaging solution Accutase Subculturing Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5- 10 ml for T75 cell culture flasks).
Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely.
Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio 1:2 to 1:4 Fluid renewal 2 to 3 times per week Freeze medium CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) Handling of cryopreserved cultures The cells come deep-frozen shipped on dry ice.
Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival.
If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds.
The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath.
A small ice clump should still remain and the vial should still be cold.
From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol.
Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature).
Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant.
The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later.
Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture sectio
