Anti-Hu CD45 Alexa Fluor® 488

• Isotype Mouse IgG1 kappa
• Specificity The mouse monoclonal antibody 2D1 reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.
• Workshop HLDA III
• Application FC (QC tested)
• Application details Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.
• 4 ml) is sufficient for 100 tests.
• Reactivity Human
• Immunogen Human peripheral blood mononuclear cells
• Preparation Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Formulation Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Storage and handling Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Description:

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes.

CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains.

These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell.

Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

A4-160-T100 Anti-Hu CD45 Alexa Fluor® 488

2D1

RUO

FC

100 tests

A6-160-T100 Anti-Hu CD45 Alexa Fluor® 647

2D1

RUO

FC

100 tests

A7-160-T100 Anti-Hu CD45 Alexa Fluor® 700

2D1

RUO

FC

100 tests

1A-160-T100 Anti-Hu CD45 APC

2D1

RUO

FC

100 tests

T4-160-T100 Anti-Hu CD45 APC-Cy™7

2D1

RUO

FC

100 tests

1F-160-T100 Anti-Hu CD45 FITC

2D1

RUO

FC

100 tests

PB-160-T100 Anti-Hu CD45 Pacific Blue™

2D1

RUO

FC

100 tests

PO-160-T100 Anti-Hu CD45 Pacific Orange™

2D1

RUO

FC

100 tests

1P-160-T100 Anti-Hu CD45 PE

2D1

RUO

FC

100 tests

T8-160-T100 Anti-Hu CD45 PE-Cy™5

2D1

RUO

FC

100 tests

T7-160-T100 Anti-Hu CD45 PE-Cy™7

2D1

RUO

FC

100 tests

PC-160-T100 Anti-Hu CD45 PerCP

2D1

RUO

FC

100 tests