Sortase A Assay Kit

Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall, maintaining bacterial virulence and infectivity. Sortase A cleaves a specific peptide sequence (LPXTG recognition motif) within a target protein between threonine and glycine. The cysteine residue of the active site forms a transient thioacyl intermediate complex with the substrate protein. This intermediate complex and substrate is then immediately attacked by oligo-glycine nucleophiles present on peptide-glycans of the bacterial wall to form an amide bond.

Since Sortase A is a critical enzyme needed to maintain the infectivity of the bacterium, it represents a promising therapeutic target. Especially for the treatment of infections caused by antibiotic-resistant bacteria.