RdRp (SARS-CoV-2) Homogeneous Assay Kit

The SARS-CoV-2 RdRp Homogeneous Assay Kit is designed to measure the activity of the SARS-CoV-2 RNA-dependent RNA Polymerase (RdRp) for screening and profiling applications. RdRp operates as a complex of NSP12, NSP7, and NSP8 proteins. RNA-dependent RNA Polymerase is a crucial enzyme in the life cycle of coronaviruses and represents one of the most promising druggable targets for SARS-CoV-2. The SARS-CoV-2 RdRp Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with reaction mixture containing Digoxigenin-labeled RNA duplex and biotinylated ATP, RdRp assay buffer (2 components plus DTT), and purified RdRp. SARS-CoV-2 RdRp Homogeneous Assay Kit measures direct incorporation of ATP in the double-stranded RNA chain. The increase in Alpha-counts is proportional to the amount of ATP incorporated in the RNA. With this kit, only three simple steps on a microtiter plate are required for RdRp activity detection. First, a sample containing RdRp enzyme is incubated with the reaction mixture. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.