Anti-PCNA PE
- Isotype
Mouse IgG2a kappa
- Specificity
The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.
- Application
- Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
- Reactivity
Mouse, Human, Non-human primates, Drosophila, Chicken, Rat
- Immunogen
recombinant rat PCNA
- Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Description
PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.
| Vendor | EXBIO Praha A.S., Czech Republik |
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