SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway)
Supplier: BPS Bioscience
Cat no.: 79806
Amount: 500 µl x 2
Applications:
- Screen for activators or inhibitors of the TGFβ/SMAD signaling pathway in transduced target cells.
- Generation of SBE Luciferase Reporter stable cell line
Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
Storage/Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 μl x 2) of SBE luciferase reporter lentivirus at a titer ≥5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
The SBE Luciferase Reporter Lentivirus (TGFβ/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized SBE-responsive element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the TGFβ/SMAD signaling pathway can be monitored by measuring the luciferase activity.
Product information: SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway) (bpsbioscience.com)
Vendor | BPS Bioscience |
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