Supplier: BPS Bioscience
Cat no.: 79806
Amount: 500 µl x 2
- Screen for activators or inhibitors of the TGFβ/SMAD signaling pathway in transduced target cells.
- Generation of SBE Luciferase Reporter stable cell line
Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
Storage/Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 μl x 2) of SBE luciferase reporter lentivirus at a titer ≥5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
The SBE Luciferase Reporter Lentivirus (TGFβ/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized SBE-responsive element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the TGFβ/SMAD signaling pathway can be monitored by measuring the luciferase activity.
Lentiviruses, a subset of retroviruses, have a storied history, with their most infamous member being the Human Immunodeficiency Virus (HIV). Historically, lentiviruses were studied for their role in disease but have since been repurposed as valuable tools for gene delivery in research due to their ability to integrate into the host genome. Parallelly, luciferase, an enzyme originally isolated from fireflies, has long been used as a bioluminescent reporter in biological studies. Its unique property to emit light upon substrate interaction made it instrumental in monitoring gene expression. Combining lentivirus vectors with luciferase reporters enabled researchers to trace and study gene expression dynamics within living organisms.
Product information: SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway) (bpsbioscience.com)