EcoPlex™ Human Inflammation 10-Plex Panel Kit (MPH003)

Introduction
EcoPlexTM Human Inflammation 10-Plex Panel Kit is based on
microsphere-based detection technology, which can
simultaneously measure multiple target proteins and with only 15
μL sample usage. This kit is for detection of IFN-γ, IL-1α, IL-1β,
IL-6, IL-8, IL-10, IL-12p70, IL-18, MCP-1 and TNF-α in human
serum, plasma, biological fluid and cell culture supernatant.
Detection Principle
EcoPlexTM kits utilize antibodies-conjugated beads which are
different in size and mean fluorescent intensity of labeling dye to
capture antigens in sample. After capture beads and sample
incubation, biotin-conjugated detection antibodies are added to
react with antigen-beads complexes, followed by a streptavidin-
R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of
PE on each bead populations is quantified by flow cytometer with
either a single 488nm laser or dual 488nm and 633/640nm lasers.
The maximum emission of the bead classification dye is at
700nm. The concentrations of protein interested are determined
by comparing the fluorescent intensities to those of standard
curve, like sandwich ELISA data analysis.
Detection Sample Types
 serum  EDTA plasma  cell culture supernatant
 other biological fluids