DMEM, w: 4.5 g/L Glucose, w: stable Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3, Very Low Endotoxin
Cat-no : P04-04515
Size: 500 ml
Store at: +2°C – +8°C
Sterile : Yes
History and Usage of DMEM
Dulbecco’s Modified Eagle Medium (DMEM HIGH GLUCOSE) stands as one of the most widely utilized media in tissue culture experiments. Developed with modifications to cater to diverse cell types, DMEM HIGH GLUCOSE is a particular variant that is vital for numerous cell culture applications.
One of the primary reasons for the popularity of DMEM HIGH GLUCOSE in tissue culture is its rich composition. Formulated with a balanced mixture of amino acids, salts, and vitamins, DMEM HIGH GLUCOSE provides cells with the essential nutrients for growth and maintenance. However, certain cells, especially rapidly dividing ones, require a higher amount of energy than others. This is where DMEM HIGH GLUCOSE becomes critical.
High glucose concentrations in DMEM HIGH GLUCOSE cater to the elevated energy demands of such cells. Glucose serves as a primary energy source for cells, being metabolized in the process of glycolysis to produce ATP, the molecular currency of energy. In tissue culture experiments, cells are often subjected to various stresses, manipulations, and conditions that might elevate their metabolic rate. Using DMEM HIGH GLUCOSE ensures that cells do not suffer from energy deprivation during these experiments.
Furthermore, DMEM HIGH GLUCOSE supports the synthesis of nucleotides, amino acids, and lipids, all crucial components as cells proliferate and function. Some specialized cells, like mammalian cell lines used in protein production, particularly benefit from high glucose conditions as they actively synthesize large amounts of proteins.
However, while using DMEM HIGH GLUCOSE, it’s vital for researchers to monitor their cultures diligently. Excess glucose can sometimes lead to a phenomenon called the “Warburg effect,” where cells predominantly utilize glycolysis over oxidative phosphorylation, even in the presence of oxygen. This shift in metabolism, while beneficial in some contexts, can alter cellular behavior.
In conclusion, DMEM with high glucose offers a specialized medium tailored for cells with high energy demands. Its usage in tissue culture experiments ensures that cells remain energetic, viable, and functionally optimal. The addition of high glucose to DMEM HIGH GLUCOSE underscores the need to understand and cater to the nuanced requirements of different cell types in culture.