Product name : Human Anti-Haemophilus influenzae type b IgG ELISA
Supplier : Xpressbio,USA
Cat no. : IM-218H
Prices start : -
Haemophilus influenzae type b can cause bacteremia, pneumonia, and acute bacterial meningitis in infants and young children. Vaccines have been developed with Haemophilus influenzae type b polysaccharide conjugated to a protein to protect from infection. Principle of the Assay Microtiter wells coated with Haemophilus influenzae type b oligosaccharideconjugated to human serum abumin (HbOHA) are exposed to test specimens, which may contain Human IgG reactive antibodies. After an incubation period, unbound components in the test sample are washed away. Specific bound Human IgG react with an anti-Human IgG conjugated with HRP during a second incubation period. Following a second wash cycle, specific bound enzyme conjugate is detected by reaction with the substrate solution, 2,2' Azino-di[3-ethyl-benzthiazolinesulfonate (ABTS). The assay is measured spectrophotometrically to indicate the level of Human AntiHaemophilus influenzae type b antibodies present in a sample.